Journal: Foods

Article Title: Photodynamic Inactivation of Foodborne Bacteria: Screening of 32 Potential Photosensitizers

doi: 10.3390/foods13030453

Figure Lengend Snippet: The minimum bactericidal concentration (MBC) and minimum biofilm eradication concentration (MBEC) values (in % w / v ) of the selected photosensitizers, based on the turbidimetric assay for S. enterica , MRSA, P. fragi , and B. thermosphacta .

Article Snippet: Choleraesuis (ATCC 10708), methicillin-resistant Staphylococcus aureus (MRSA 456 S. Yanke), Pseudomonas fragi (ATCC 4973), and Brochothrix thermosphacta (ATCC 11509) were used in this study.

Techniques: Concentration Assay, Turbidimetric Assay

Journal: Journal of medicinal chemistry

Article Title: Consideration of Binding Kinetics in the Design of Stapled Peptide Mimics of the Disordered Proteins Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 and Eukaryotic Translation Initiation Factor 4G.

doi: 10.1021/acs.jmedchem.9b00068

Figure Lengend Snippet: Figure 1. Regulation of cap-dependent translation initiation by 4E- BP1 and eIF4G. (A) Biological scheme. (B) Overlay and amino acid sequences of the eIF4E-binding regions for 4E-BP1 (blue; PDB: 4UED) and eIF4GI (green; PDB: 5T46) bound to eIF4E (gray).

Article Snippet: 2019, 62, 4967−4978 4973 eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Binding Assay

Journal: Journal of medicinal chemistry

Article Title: Consideration of Binding Kinetics in the Design of Stapled Peptide Mimics of the Disordered Proteins Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 and Eukaryotic Translation Initiation Factor 4G.

doi: 10.1021/acs.jmedchem.9b00068

Figure Lengend Snippet: Figure 3. Kinetic analyses of 4E-BP1, 4E-BP1 (R51−N64), and eIF4G peptides via SPR. The effect of temperature on (A) ka, (B) kd and (C) Kd. Statistical significance was determined using unpaired, two-tailed Student’s t tests. ns = not significant (p > 0.05). (A) ***: p = 0.0004. (B) ****: p = <0.0001; **: p = 0.0015. (C) *: p = 0.0162; ***: p = 0.0005; ****: p = <0.0001. Please see Tables S1−S3 for tabulated results, p values and statistical analyses.

Article Snippet: 2019, 62, 4967−4978 4973 eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Two Tailed Test

Journal: Journal of medicinal chemistry

Article Title: Consideration of Binding Kinetics in the Design of Stapled Peptide Mimics of the Disordered Proteins Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 and Eukaryotic Translation Initiation Factor 4G.

doi: 10.1021/acs.jmedchem.9b00068

Figure Lengend Snippet: Figure 4. Kinetic analyses of 4E-BP1, 4E-BP1 (R51−N64), and eIF4G peptides via SPR. The effect of ionic strength at 25 °C on (A) ka, (B) kd, and (C) Kd. Statistical significance was determined using unpaired, two-tailed Student’s t tests. ns = not significant (p > 0.05). (A) **: p = 0.0025; ***: p = 0.0002. (B) **: p = 0.0023. (C) *: p = 0.0161; **: p = 0.0024 and 0.0041, respectively. Please see Tables S4−S6 for tabulated results, p values, and statistical analyses. In (A), the error bar for eIF4G is too small to see (Table S4).

Article Snippet: 2019, 62, 4967−4978 4973 eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Two Tailed Test

Journal: Journal of medicinal chemistry

Article Title: Consideration of Binding Kinetics in the Design of Stapled Peptide Mimics of the Disordered Proteins Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 and Eukaryotic Translation Initiation Factor 4G.

doi: 10.1021/acs.jmedchem.9b00068

Figure Lengend Snippet: Figure 5. Stapled peptide helicities as determined via CD spectros- copy in 5 mM sodium phosphate buffer, pH 7.4, 25 °C. (A) 4E-BP1 and HCS-4E-BP1 spectra. (B) eIF4G and HCS-eIF4G spectra. (C) Linear and sTIP-04 spectra. (D) Table of average helicity values measured at 25−100 μM ± standard deviation across the concentrations tested. For the TFE experiments, only 100 μM peptide was used.

Article Snippet: 2019, 62, 4967−4978 4973 eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Standard Deviation

Journal: Journal of medicinal chemistry

Article Title: Consideration of Binding Kinetics in the Design of Stapled Peptide Mimics of the Disordered Proteins Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 and Eukaryotic Translation Initiation Factor 4G.

doi: 10.1021/acs.jmedchem.9b00068

Figure Lengend Snippet: Figure 6. In vitro inhibitory activities of HCS-4E-BP1 and -eIF4G peptides as determined via PPI cat-ELCCA. (A) Inhibition of the eIF4E−4E-BP1 PPI by 4E-BP1, HCS-4E-BP1, and HCS-4E-BP1 (LMAA) peptides; and (B) eIF4G, HCS-eIF4G, linear sTIP-04, and sTIP-04 peptides. (C) Table of IC50 values presented as 95% confidence intervals.

Article Snippet: 2019, 62, 4967−4978 4973 eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: In Vitro, Inhibition

Journal: Journal of medicinal chemistry

Article Title: Consideration of Binding Kinetics in the Design of Stapled Peptide Mimics of the Disordered Proteins Eukaryotic Translation Initiation Factor 4E-Binding Protein 1 and Eukaryotic Translation Initiation Factor 4G.

doi: 10.1021/acs.jmedchem.9b00068

Figure Lengend Snippet: Figure 7. Cellular inhibitory activities of the sTIP-04, HCS-4E-BP1, and HCS-4E-BP1 (LMAA) peptides as determined via m7GDP cap affinity chromatography. (A) Assay scheme. (B) Inhibition of the eIF4E−4E-BP1 and eIF4E−eIF4G PPIs in MDA-MB-231 cells. In all cases, cells were treated with peptides dissolved in water for 6 h and eIF4E was used as a normalization control. (C) Quantitation of the HCS-4E-BP1 data in (B) from triplicate biological replicates. (D) Quantitation of the sTIP-04 data in (B) from triplicate biological replicates. Please see Tables S9 and S10 for p values.

Article Snippet: 2019, 62, 4967−4978 4973 eIF4G (2858) antibodies were purchased from Cell Signaling Technology.

Techniques: Chromatography, Inhibition, Control, Quantitation Assay